Catalog Number：RA10140
Epsilon Tubulin Mouse Monoclonal Antibody(2B3)
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Size：
50 μL
100 μL
Price：
$149.00
$249.00
Delivery： Order now, ship in 3 days
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| Product Name | Epsilon Tubulin Mouse Monoclonal Antibody(2B3) | Species | H,M,R |
| Applications | WB | ||
| Condition | 1mg/ml in PBS, pH 7.4, containing 0.02% sodium azide and 50% glycerol. | ||
| Alternative Names | FLJ22589,TUBE1,tubulin epsilon 1,ε tubulin | ||
| Store | Store at -20°C. Stable for one year from the date of shipment. | ||
| Recommended dilutions | WB 1:1,000-2,000 | ||
| Specificity | Antibody can detect endogenous Epsilon tubulin proteins. | ||
| Background | Epsilon-tubulin (ε-tubulin) is a less-characterized member of the tubulin superfamily, which includes alpha (α), beta (β), gamma (γ), delta (δ), and epsilon (ε) isoforms. While α- and β-tubulins form microtubule polymers critical for cell structure and transport, ε-tubulin is primarily localized to centrioles and basal bodies, playing a role in centriole duplication, cilia formation, and mitotic spindle organization. It is evolutionarily conserved but exhibits functional divergence across species. For instance, in mammals, ε-tubulin interacts with other centriolar proteins like γ-tubulin and is essential for maintaining centrosome integrity. Its dysregulation has been linked to ciliopathies, microcephaly, and cancer. Antibodies targeting ε-tubulin are vital tools for studying centriole biology and cell cycle dynamics. They enable visualization of centriolar structures via immunofluorescence, Western blotting, or immunoprecipitation, aiding research on centrosome amplification in tumors or ciliary defects. However, ε-tubulin antibodies require rigorous validation due to high sequence homology among tubulin isoforms. Specificity is often confirmed using knockout cell lines or siRNA knockdown. Commercial antibodies are typically raised against conserved C-terminal epitopes, with reactivity tested in human, mouse, or rat models. Recent studies also employ these antibodies to explore ε-tubulin's non-canonical roles, such as in DNA damage response or cellular senescence, highlighting its expanding significance in cell biology and disease mechanisms. | ||
| Molecular Weight | 55KD | Isotype | IgG1 |
| GeneID | 51175 | Protein MW | 55KD |
| SWISS | Q9UJT0 | Source | Mouse |
| Antigen | Synthetic Peptide | Category | Monoclonal |
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Western blot analysis of 1)MCF7Cell Lysate, 2)Rat Brain Tissue Lysate, 3)Mouse Brain Tissue Lysate using Epsilon Tubulin Mouse mAb diluted at 1:2000.