TECHNOLOGY

Q1: What is the role of plate washing?​​ To remove unbound antigens, antibodies, and other impurities, reduce non-specific reactions, and improve detection sensitivity, specificity, and accuracy.   Q2: How to prepare the wash buffer?​​ The kit-supplied wash buffer is a 30 mL, 25X co

1. How to avoid hemolysis during blood collection? The main causes of hemolysis: ① Mechanical damage: too slow or too fast blood collection speed, violent mixing, too thin needle (

1. Which Anticoagulants Can Be Used When Preparing Plasma Samples for ELISA?​​ For plasma preparation, EDTA salt anticoagulants are recommended (e.g., EDTA2Na, concentration: 1.5–2.2 mg/mL blood). Pre-filled collection tubes typically contain 4.5 mL blood + 7.2 mg EDTA2N

1. Why Is BCA Total Protein Quantification Required for ELISA Detection of Tissue Homogenate Supernatant and Cell Lysates?​​ ① Reduce Errors: Variability in sample volume and protein extraction efficiency during tissue homogenization/cell lysis can lead to inconsistencies. BCA quantification e

1. Why Use Protease Inhibitors When Preparing Tissue Homogenates or Cell Lysates for ELISA?​​ When preparing tissue homogenates or cell lysates, endogenous proteases (e.g., serine proteases, cysteine proteases, metalloproteases) are released, which can cause: ① Target Protein Degradation: Degr