TECHNOLOGY

1. Sample Processing methods in special circumstances Regarding high-fat samples：High-fat samples contain fat and are not homogeneous solutions,  direct loading will affect the binding of antigen and antibody, resulting in inaccurate measurement results.It is recommended to centrifuge a

Please predict the concentration range of the sample in advance. If your samples need to be diluted, please refer to the following dilution instructions: For 100 folds dilution: One step dilution. Add 5μL sample to 495μL sample diluent to yield 100 folds dilution. For 1000 folds dilution

When performing an ELISA assay, one of the most challenging steps is often not pipetting—it’s standard curve fitting. The kit manual may recommend using a four-parameter logistic (4PL) model, but when you open Excel, a linear regression can also give you an impressive R² = 0.98. So

Respiratory inflammatory conditions such as pneumonia, influenza, allergic rhinitis, and mild infections surge during seasonal transitions, making inflammatory cytokine quantification a major challenge for researchers and clinical laboratory professionals. Traditional ELISA kits perform adeq

In the foundational chapter of our protein quantification series, we classified mainstream detection techniques into two core categories and illustrated the fundamental principles of non-specific total protein assays (UV absorbance, BCA assay, and Coomassie Brilliant Blue method) and conventional si

When performing ELISA assays, many researchers encounter a common issue: the optical density (OD) values of some samples are excessively high and exceed the maximum OD value of the standard curve. Some researchers simply discard the data and reload samples; others dilute samples arbitrarily and calc