TECHNOLOGY

Standard Curve FAQ Q1: Why are the standards in ELISA kits made into lyophilized powder instead of pre-diluted liquid standard solutions? A: The lyophilization process (freeze-drying) dehydrates and freezes protein solutions at low temperatures, which can maximize the preservation of biologica

ELISA Procedures  - Reconstitution and Dilution of Standards In ELISA (Enzyme-Linked Immunosorbent Assay), the quality of the standard curve determines the reliability and accuracy of experimental results directly. An ideal standard curve not only requires a good linear rel

Q1: What is the role of plate washing?​​ To remove unbound antigens, antibodies, and other impurities, reduce non-specific reactions, and improve detection sensitivity, specificity, and accuracy.   Q2: How to prepare the wash buffer?​​ The kit-supplied wash buffer is a 30 mL, 25X co

1. How to avoid hemolysis during blood collection? The main causes of hemolysis: ① Mechanical damage: too slow or too fast blood collection speed, violent mixing, too thin needle (

1. Which Anticoagulants Can Be Used When Preparing Plasma Samples for ELISA?​​ For plasma preparation, EDTA salt anticoagulants are recommended (e.g., EDTA2Na, concentration: 1.5–2.2 mg/mL blood). Pre-filled collection tubes typically contain 4.5 mL blood + 7.2 mg EDTA2N

1. Why Is BCA Total Protein Quantification Required for ELISA Detection of Tissue Homogenate Supernatant and Cell Lysates?​​ ① Reduce Errors: Variability in sample volume and protein extraction efficiency during tissue homogenization/cell lysis can lead to inconsistencies. BCA quantification e