TECHNOLOGY

When performing ELISA assays, many researchers encounter a common issue: the optical density (OD) values of some samples are excessively high and exceed the maximum OD value of the standard curve. Some researchers simply discard the data and reload samples; others dilute samples arbitrarily and calc

Researchers conducting ELISA experiments have likely encountered these frustrating moments: Failure to detect low-concentration samples at all, with abnormally high values in blank wells; Poor standard curve fitting, substandard correlation coefficients, leading to questioned research data; Sever

In the life sciences, protein quantification is a frequently used research technique. Many researchers have faced this common dilemma: with more than a dozen protein quantification methods available—ELISA, BCA, chemiluminescence, and more—which one should you choose? Reed Biotech&

In ELISA tests, serum and plasma are the most widely used sample types. Differences in sample type, sample preprocessing, and the selection of blood collection tubes can directly affect the accuracy, stability, and repeatability of the assay results. This article focuses on the key differences betwe

Three Soul-searching Questions 1. Does the OD value difference between duplicate wells on the same plate exceed 0.2? 2. Does the gradient dilution cause more fluctuations? 3. Did the standard curve fit R² = 0.94, and the paper was rejected by the reviewers? Don't rush to blame the kit

Standard Curve FAQ Q1: Why are the standards in ELISA kits made into lyophilized powder instead of pre-diluted liquid standard solutions? A: The lyophilization process (freeze-drying) dehydrates and freezes protein solutions at low temperatures, which can maximize the preservation of biologica