TECHNOLOGY

When performing an ELISA assay, one of the most challenging steps is often not pipetting—it’s standard curve fitting. The kit manual may recommend using a four-parameter logistic (4PL) model, but when you open Excel, a linear regression can also give you an impressive R² = 0.98. So

Respiratory inflammatory conditions such as pneumonia, influenza, allergic rhinitis, and mild infections surge during seasonal transitions, making inflammatory cytokine quantification a major challenge for researchers and clinical laboratory professionals. Traditional ELISA kits perform adeq

In the foundational chapter of our protein quantification series, we classified mainstream detection techniques into two core categories and illustrated the fundamental principles of non-specific total protein assays (UV absorbance, BCA assay, and Coomassie Brilliant Blue method) and conventional si

When performing ELISA assays, many researchers encounter a common issue: the optical density (OD) values of some samples are excessively high and exceed the maximum OD value of the standard curve. Some researchers simply discard the data and reload samples; others dilute samples arbitrarily and calc

Researchers conducting ELISA experiments have likely encountered these frustrating moments: Failure to detect low-concentration samples at all, with abnormally high values in blank wells; Poor standard curve fitting, substandard correlation coefficients, leading to questioned research data; Sever

In the life sciences, protein quantification is a frequently used research technique. Many researchers have faced this common dilemma: with more than a dozen protein quantification methods available—ELISA, BCA, chemiluminescence, and more—which one should you choose? Reed Biotech&