Catalog Number：RTA21
Flag-Tag Mouse Monoclonal Antibody(2C5)
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Size：
50 μL
100 μL
Price：
$149.00
$249.00
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| Product Name | Flag-Tag Mouse Monoclonal Antibody(2C5) | Species | N/A |
| Applications | WB,IF,IP | ||
| Condition | 1mg/ml in PBS, pH 7.4, containing 0.02% sodium azide and 50% glycerol. | ||
| Alternative Names | |||
| Store | Store at -20°C. Stable for one year from the date of shipment. | ||
| Recommended dilutions | WB 1:5,000 IHC 1:200 IF 1:2,000 | ||
| Specificity | Antibody can recognize C-terminal, internal, and N-terminal Flag-tag fusion proteins. | ||
| Background | The DYKDDDDK peptide (Flag-tag) is a small component of an epitope which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein. It has been used extensively as a general epitope tag in expression vectors. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.A Flag-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a Flag-tag to this protein allows one to follow the protein with an antibody against the Flag sequence. | ||
| Molecular Weight | N/A | Isotype | IgG1 |
| GeneID | N/A | Protein MW | N/A |
| SWISS | N/A | Source | Mouse |
| Antigen | Synthetic Peptide | Category | Monoclonal |
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1ug Flag fusion protein+ Primary antibody dilution at 1:10,000.
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IF analysis of 293 cells transfected with a Flag-tag protein, using anti-FlagTag Mouse mAb at a 1:2000 dilution (blue DAPI, red anti-Flag)