Catalog Number：RCA41
Flag Mouse Monoclonal Antibody(2C5)-HRP conjugated
Copy Product Information
Size：
50 μL
100 μL
Price：
$99.00
$129.00
Delivery： Order now, ship in 3 days
Manual DownloadProduct Details
| Product Name | Flag Mouse Monoclonal Antibody(2C5)-HRP conjugated | Species | N/A |
| Applications | WB | ||
| Condition | PBS, pH 7.4, containing 0.02% Kathon as Preservative and 50% Glycerol. | ||
| Alternative Names | DDDDK epitope tag, DYKDDDDK epitope tag, ECS epitope tag | ||
| Store | -20°C. Do not aliquot the antibody. | ||
| Recommended dilutions | WB: 1:5,000-10,000 Optimal dilutions should be determined by the end user. | ||
| Specificity | The Flag tag antibody can recognize C-terminal, internal, and N-terminal Flag-tag fusion proteins. | ||
| Background | The DYKDDDDK peptide (Flag-tag) is a small component of an epitope which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein. It has been used extensively as a general epitope tag in expression vectors. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.A Flag-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a Flag-tag to this protein allows one to follow the protein with an antibody against the Flag sequence. | ||
| Molecular Weight | N/A | Isotype | IgG1 |
| GeneID | N/A | Protein MW | N/A |
| SWISS | N/A | Source | Mouse |
| Antigen | Synthetic Peptide | Category | Monoclonal |
-
Western blot analysis of Recombinant protein with RCA41 diluted at 1) 1:5,000 2) 1:10,000