Catalog Number：RP0071
Recombinant Human IL-4 (E. coli)
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Size：
50 μg
500 μg
Price：
$560.00
$1968.00
Delivery： Order now, ship in 3 days
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| Product Name | Recombinant Human IL-4 (E. coli) | Accesstion Number | P05112 |
| Alternative Names | Interleukin-4; IL-4; B-Cell Stimulatory Factor 1; BSF-1; Binetrakin; Lymphocyte Stimulatory Factor 1; Pitrakinra; IL4 | ||
| Description | Recombinant Human Interleukin-4 is produced by our E.coli expression system and the target gene encoding His25-Ser153 is expressed. | ||
| Calculated Molecular Weight | Mol Mass:15.1 KDa; APMol Mass:13-14 KDa, reducing conditions | Formulation | Lyophilized from a 0.2 μm filtered solution of 20mM Tris-HCl, 150mM NaCl, pH 7.2. |
| Endotoxin | < 0.01 EU/µg as determined by LAL test. | Purity | Greater than 95% as determined by reducing SDS-PAGE. (QC verified) |
| Biological Activity | Measured in a cell proliferation assay using TF‑1 human erythroleukemic cells. The ED50 for this effect is 10-70 pg/ml. (QC verified) | ||
| Reconstitution | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. | ||
| Storage | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. | ||
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature listed below. | ||
| Background | Interleukin-4 (IL-4) is a pleiotropic cytokine that regulates diverse T and B cell responses including cell proliferation, survival and gene expression. IL-4 is produced by mast cells, T cells, and bone marrow stromal cells. IL-4 regulates the differentiation of naive CD4+ T cells into helper Th2 cells, characterized by their cytokine-secretion profile that includes secretion of IL-4, IL-5, IL-6, IL-10, and IL-13, which favor a humoral immune response. Another dominant function of IL-4 is the regulation of immunoglobulin class switching to the IgG1 and IgE isotypes. Excessive IL-4 production by Th2 cells has been associated with elevated IgE production and allergic response. | ||
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Greater than 95% as determined by reducing SDS-PAGE. (QC verified)
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Measured in a cell proliferation assay using TF‑1 human erythroleukemic cells. The ED50 for this effect is 10-70 pg/ml. (QC verified)