Catalog Number：RP0066
Recombinant Human Beta-NGF
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Size：
50 μg
500 μg
Price：
$240.00
$1792.00
Delivery： Order now, ship in 3 days
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| Product Name | Recombinant Human Beta-NGF | Accesstion Number | P01138 |
| Alternative Names | Beta-Nerve Growth Factor; Beta-NGF; NGF; NGFB;β-NGF | ||
| Description | Recombinant Human Beta-Nerve Growth Factor is produced by our E.coli expression system and the target gene encoding Ser122-Ala241 is expressed. | ||
| Calculated Molecular Weight | Mol Mass:13.4 KDa; APMol Mass:14 KDa, reducing conditions | Formulation | Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4. |
| Endotoxin | < 0.01 EU/µg as determined by LAL test. | Purity | Greater than 95% as determined by reducing SDS-PAGE. (QC verified) |
| Biological Activity | Measured in a cell proliferation assay using TF‑1 human erythroleukemic cells. The ED50 for this effect is 0.03-0.3 ng/ml. (QC verified) | ||
| Reconstitution | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. | ||
| Storage | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. | ||
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature listed below. | ||
| Background | Human β-Nerve Growth Factor (β-NGF) was initially isolated in the mouse submandibular gland. It is composed of three non-covalently linked subunits α, β, and γ; it exhibits all the biological activities ascribed to NGF. It is structurally related to BDNF, NT-3 and NT-4 and belongs to the cysteine-knot family of growth factors that assume stable dimeric structures. Β-NGF is a neurotrophic factor that signals through its receptor β-NGF, and plays a crucial role in the development and preservation of the sensory and sympathetic nervous systems. Β-NGF also acts as a growth and differentiation factor for B lymphocytes and enhances B-cell survival. These results suggest that β-NGF is a pleiotropic cytokine, which in addition to its neurotropic activities may have an important role in the regulation of the immune system. Human β-NGF shares 90% sequence similarity with mouse protein and shows cross-species reactivity. | ||
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Greater than 95% as determined by reducing SDS-PAGE. (QC verified)
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Measured in a cell proliferation assay using TF‑1 human erythroleukemic cells. The ED50 for this effect is 0.03-0.3 ng/ml. (QC verified)