Catalog Number：RP0058
Recombinant Mouse IL-7 (C-6His)
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Size：
50 μg
500 μg
Price：
$704.00
$2464.00
Delivery： Order now, ship in 3 days
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| Product Name | Recombinant Mouse IL-7 (C-6His) | Accesstion Number | P10168 |
| Alternative Names | IL-7; IL-7 interleukin-7; interleukin-7; Lymphopoietin -1; PBGF | ||
| Description | Recombinant Mouse Interleukin-7 is produced by our Mammalian expression system and the target gene encoding Glu26-Ile154 is expressed with a 6His tag at the C-terminus. | ||
| Calculated Molecular Weight | Mol Mass:15.9 KDa; APMol Mass:22-28 KDa, reducing conditions | Formulation | Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4. |
| Endotoxin | < 1 EU/µg as determined by LAL test. | Purity | Greater than 95% as determined by reducing SDS-PAGE. (QC verified) |
| Biological Activity | Measured in a cell proliferation assay using PHA-activated human peripheral blood lymphocytes (PBL).The ED50 for this effect is 60-1000 pg/ml. (QC verified) | ||
| Reconstitution | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. | ||
| Storage | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. | ||
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature listed below. | ||
| Background | Mouse interleukin-7(IL-7) is the member of hemopoietin family which is important to the differentiation, proliferation, and survival of lymphocyte. Mouse IL-7 shares approximately 88% aa sequence identity with rat IL-7 and 58-60% with human, equine, bovine, ovine, porcine, feline and canine IL-7. It is widely expressed in primary and secondary lymphoid tissues cell and stromal epithelial cells of the thymus, bone marrow, and intestines. IL-7 activation of IL-7 R alpha is critical for both T cell and B cell lineage development. It is important for proliferation during certain stages of B-cell maturation. IL-7 contributes to the maintenance of all naïve and memory T cells, mainly by promoting expression of the anti-apoptotic protein Bcl-2. It is required for optimal T cell-dendritic cell interaction. | ||
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Greater than 95% as determined by reducing SDS-PAGE. (QC verified)
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Measured in a cell proliferation assay using PHA-activated human peripheral blood lymphocytes (PBL).The ED50 for this effect is 60-1000 pg/ml. (QC verified)