Catalog Number：RP0053
Recombinant Human IFN gamma (E. coli)
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Size：
50 μg
500 μg
Price：
$160.00
$504.00
Delivery： Order now, ship in 3 days
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| Product Name | Recombinant Human IFN gamma (E. coli) | Accesstion Number | P01579 |
| Alternative Names | Interferon Gamma; IFN-Gamma; Immune Interferon; IFNG | ||
| Description | Recombinant Human Interferon Gamma is produced by our E.coli expression system and the target gene encoding Gln24-Gln166 is expressed. | ||
| Calculated Molecular Weight | Mol Mass:16.88 KDa; APMol Mass:16 KDa, reducing conditions | Formulation | Lyophilized from a 0.2 μm filtered solution of 20mM PB, 5% Sucrose, 4% Mannitol, 0.02% Tween 80, pH7.4. |
| Endotoxin | < 0.01 EU/µg as determined by LAL test. | Purity | Greater than 95% as determined by reducing SDS-PAGE. (QC verified) |
| Biological Activity | Measured by a cytotoxicity assay using HT-29 cells. The ED50 for this effect is 17 pg/ml. (Regularly tested) | ||
| Reconstitution | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. | ||
| Storage | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. | ||
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature listed below. | ||
| Background | IFNγ is the major interferon produced by mitogenically or antigenically stimulated lymphocytes. It is structurally different from type I interferon and its major activity is immunoregulation. It has been implicated in the expression of class II histocompatibility antigens in cells that do not normally produce them, leading to autoimmune disease. Interferon gamma is produced mainly byT-cells and natural killer cells activated by antigens, mitogens, or alloantigens. It is produced by lymphocytes expressing the surface antigens CD4 and CD8. IFNγ synthesis is induced by IL-2, FGF-basic, and EGF. | ||
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Greater than 95% as determined by reducing SDS-PAGE. (QC verified)
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Measured by a cytotoxicity assay using HT-29 cells. The ED50 for this effect is 17 pg/ml. (Regularly tested)