Catalog Number：RP0007
Recombinant Human/Mouse/Rat Activin A
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Size：
50 μg
500 μg
Price：
$704.00
$3584.00
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| Product Name | Recombinant Human/Mouse/Rat Activin A | Accesstion Number | P08476 |
| Alternative Names | Inhibin beta A chain; INHBA; Activin A | ||
| Description | Recombinant Human Activin A is produced by our Mammalian expression system and the target gene encoding Gly311-Ser426 is expressed. | ||
| Calculated Molecular Weight | Mol Mass:13 KDa; APMol Mass:15 KDa, reducing conditions | Formulation | Lyophilized from a 0.2 μm filtered solution of 4mM HCl. |
| Endotoxin | < 0.01 EU/µg as determined by LAL test. | Purity | Greater than 95% as determined by reducing SDS-PAGE. (QC verified) |
| Biological Activity | Measured by its ability to induce SMAD signaling in 293-Activin A Res cells. The ED50 for this effect is 1.3 ng/ml. (Regularly tested)|Human intestinal organoids (ipsc source) were cultured with Activin A , BMP-4 , EGF , Wnt3a , Noggin, R-spondin 1 and FGF-4 . The organoids showed good morphology. | ||
| Reconstitution | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. | ||
| Storage | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. | ||
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature listed below. | ||
| Background | Activin and inhibin are two closely related protein complexes that have almost directly opposite biological effects. Activins, members of the TGF-beta superfamily, are disulfide-linked dimeric proteins originally purified from gonadal fluids as proteins that stimulated pituitary follicle stimulating hormone (FSH) release. Inhibins/activins are involved in regulating a number of diverse functions such as hypothalamic and pituitary hormone secretion, gonadal hormone secretion, germ cell development and maturation, erythroid differentiation, insulin secretion, nerve cell survival, embryonic axial development or bone growth, depending on their subunit composition. Activins are homodimers or heterodimers of the various beta subunit isoforms, while inhibins are heterodimers of a unique alpha subunit and one of the various beta subunits. | ||
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Greater than 95% as determined by reducing SDS-PAGE. (QC verified)
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Measured by its ability to induce SMAD signaling in 293-Activin A Res cells. The ED50 for this effect is 1.3 ng/ml (Regularly tested).