Catalog Number：RCA24
β-actin Mouse Monoclonal Antibody (A02)
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Size：
50 μL
100 μL
Price：
$99.00
$129.00
Delivery： Order now, ship in 3 days
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| Product Name | β-actin Mouse Monoclonal Antibody (A02) | Species | H, R, M, Mk, Dg, Ch, Hm, Rb, Pg, Sh |
| Applications | WB, IHC, IF | ||
| Condition | PBS, pH 7.4, containing 0.02% sodium azide as Preservative and 50% Glycerol. | ||
| Alternative Names | actin antibody, b-actin antibody, beta-actin antibody | ||
| Store | -20°C. Do not aliquot the antibody. | ||
| Recommended dilutions | WB: 1:5,000-10,000 IHC: 1:200 IF: 1:200 Optimal dilutions should be determined by the end user. | ||
| Specificity | Antibody can detects endogenous β-actin protein. | ||
| Background | β-actin is one of six different actin isoforms that have been identified. The actin molecules found in cells of various species and tissues tend to be very similar in their immunological and physical properties. Therefore, antibodies against β-actin are useful as loading controls for Western Blotting. However it should be noted that levels of β-actin may not be stable in certain cells. For example, expression of β-actin in adipose tissue is very low and therefore β-actin should not be used as loading control for these tissues. | ||
| Molecular Weight | 43KD | Isotype | IgG1 |
| GeneID | 60 | Protein MW | 43 |
| SWISS | P60709 | Source | Mouse |
| Antigen | Synthetic Peptide | Category | Monoclonal |
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Western blot analysis of Hela (1), Rat brain (2), Mouse brain(3), with β-actin mouse mAb(A02) diluted at 1:10,000.
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IF analysis of Hela with β-actin mouse mAb(A02) diluted at 1:500.