Catalog Number：RCA22
β-Tubulin Mouse Monoclonal Antibody(5G3)
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Size：
50 μL
100 μL
Price：
$99.00
$129.00
Delivery： Order now, ship in 3 days
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| Product Name | β-Tubulin Mouse Monoclonal Antibody(5G3) | Species | H, R, M, Mk, Dg, C, Hm, Rb, Sh Insect, Yeast |
| Applications | WB, IHC, IF | ||
| Condition | PBS, pH 7.4, containing 0.02% sodium azide as Preservative and 50% Glycerol. | ||
| Alternative Names | |||
| Store | -20°C. Do not aliquot the antibody. | ||
| Recommended dilutions | WB: 1:5,000 IHC: 1:200 IF: 1:100-200 Optimal dilutions should be determined by the end user. | ||
| Specificity | Antibody can detects endogenous β-tubulin protein. | ||
| Background | Microtubules are constituent parts of the mitotic apparatus, cilia, flagella, and elements of the cytoskeleton. They consist principally of 2 soluble proteins, alpha- and beta-tubulin, each of about 55,000 Da. Antibodies against beta Tubulin are useful as loading controls for Western Blotting. However it should be noted that levels of β-Tubulin may not be stable in certain cells. For example, expression of β-Tubulin in adipose tissue is very low and therefore β-Tubulin should not be used as loading control for these tissues. | ||
| Molecular Weight | 55KD | Isotype | IgG1 |
| GeneID | 203068 | Protein MW | 55 |
| SWISS | P07437 | Source | Mouse |
| Antigen | Synthetic Peptide | Category | Monoclonal |
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Western blot analysis of A549 (1) , Rat brain (2) ,Mouse brain (3) ,Chicken lung (4) and Rabbit testis (5),Sheep muscle (6) with β-tubulin mouse mAb(5G3) diluted at 1:5000.
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IHC Staining of Human colon tissue with β-tubulin mouse mAb(5G3) diluted at 1:200.
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IF analysis of Hela with β-tubulin mouse mAb(Left) and DAPI (Right) diluted at 1:100.