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Catalog Number:RCA20
β-actin Mouse Monoclonal Antibody(5B7)
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Size:

50 μL

100 μL

Price:

$99.00

$129.00

Delivery: Order now, ship in 3 days

Product Details
Product Name β-actin Mouse Monoclonal Antibody(5B7) Species H, R, M, Mk, Dg, Ch, Hm, Rb, Pg, Sh Insect
Applications WB, IHC, IF
Condition PBS, pH 7.4, containing 0.02% sodium azide as Preservative and 50% Glycerol.
Alternative Names
Store -20°C. Do not aliquot the antibody.
Recommended dilutions WB: 1:5,000 IHC: 1:200 IF: 1:200 Optimal dilutions should be determined by the end user.
Specificity Antibody can detects endogenous β-actin protein.
Background β-Actin is one of six different actin isoforms that have been identified. The actin molecules found in cells of various species and tissues tend to be very similar in their immunological and physical properties. Therefore, Antibodies against β-Actin are useful as loading controls for Western Blotting. However it should be noted that levels of β-Actin may not be stable in certain cells. For example, expression of β-Actin in adipose tissue is very low and therefore β-Actin should not be used as loading control for these tissues.
Molecular Weight 43KD Isotype IgG1
GeneID 60 Protein MW 43
SWISS P60709 Source Mouse
Antigen Synthetic Peptide Category Monoclonal
  • Western blot analysis of Hela (1) Rat brain (2)Mouse brain (3) Chicken lung (4) and Rabbit testis (5)with β-Actin mouse mAb(5B7) diluted at 1:10,000.
  • IHC Staining of Human ovary tissue with β-Actin mouse mAb(5B7) diluted at 1:200