NEWS CENTER

Have you ever found yourself struggling with the limitations of traditional ELISA assays?

From tedious multiple washing steps and strict 100 μL sample volume requirements to complex sample dilution optimization, limited sensitivity for low-abundance targets, and lengthy assay procedures, traditional ELISA often demands significant time and effort. Not only can it consume valuable samples and reagents, but it can also introduce experimental variability, turning what should be a straightforward protein analysis into a time-consuming challenge.

To address diverse research needs, Traditional ELISA manufacturers have developed multiple kit formats. For samples with large concentration differences—such as cell culture supernatants collected before and after stimulation—researchers typically need to choose a wide-range ELISA kit. When sample volume is limited, such as mouse serum, a micro-volume ELISA kit becomes necessary. For ultra-low-abundance biomarkers, including human serum cytokines like IL-6 and TNF-α, high-sensitivity ELISA kits are often the only option.

As a result, researchers are frequently faced with a difficult decision: Which kit is the right one for their experiment?

Now, there is a better solution.

The newly launched VeriQuant™ Immunoassay Kit from Reed Biotech brings together the advantages of wide dynamic range, low sample consumption, high sensitivity, and streamlined workflow—all in a single kit.

The VeriQuant™ Immunoassay Kit is developed based on the Proximity Ligation Assay (PLA) technology. In this assay, two antibodies recognizing distinct epitopes of the target protein are each conjugated to unique oligonucleotides. When both antibodies bind simultaneously to the same target protein, the attached oligonucleotides are brought into close proximity. These oligonucleotides are then joined by synthesis enzymes to form a DNA template, which is subsequently amplified by quantitative PCR (qPCR) using specific primers and a TaqMan probe. The resulting fluorescence signal is inversely correlated with the cycle threshold (Ct) value, enabling highly specific and ultra-sensitive quantification of the target protein.

While ensuring highly accurate and reliable detection results, VeriQuant™ fundamentally redefines the traditional immunoassay workflow. Combining minimal hands-on operation, exceptional sensitivity, an ultra-wide dynamic range, and extremely low sample consumption, it significantly improves the overall experimental experience and enhances laboratory efficiency. By eliminating tedious and repetitive assay procedures, VeriQuant™ allows researchers to spend less time on routine experimentation and more time on meaningful scientific discovery and data analysis.

H1: Lightning-Fast Workflow, No Constant Monitoring Required

A traditional ELISA workflow typically involves multiple sequential steps:Standard/Sample Incubation → Plate Washing → Biotinylated Antibody Addition → Incubation → Plate Washing → SA-HRP Addition → Incubation → Plate Washing → Substrate Addition → Stop Solution Addition → Plate Reading
From start to finish, the entire process often requires more than 5 hours, including over an hour of labor-intensive hands-on work. Moreover, the assay demands continuous operator attention to monitor each reaction step and ensure timely termination and data acquisition. This not only consumes valuable time and effort but also leaves little room for error, ultimately slowing research progress.

VeriQuant™ PLA Immunoassay Kits dramatically streamline the workflow with just two simple steps:Add standards/samples and antibody conjugates for incubation → Add qPCR reaction mix and run on a qPCR instrument
The total assay time is reduced to approximately 2 hours, while hands-on operation takes less than 20 minutes.
All critical reaction steps are automatically performed by the qPCR instrument. Once the run is started, no further user intervention is required, and complete data are generated automatically at the end of the assay.
By eliminating lengthy manual procedures and constant monitoring, VeriQuant™ frees researchers from repetitive bench work, significantly increases laboratory throughput, and enables greater focus on scientific discovery rather than routine experimentation.

H2:Homogeneous Assay, Zero Washing: Eliminate the Most Tedious Step in Traditional ELISA
Anyone who has performed a traditional ELISA knows that repeated washing cycles and manual plate tapping are an unavoidable part of the workflow. These tedious and physically demanding steps not only consume valuable time but can also introduce variability. Minor inconsistencies in manual handling or incomplete washing may lead to fluctuating results, poor reproducibility, and, in the worst-case scenario, the need to repeat an entire plate.

The new VeriQuant™ Immunoassay Kit, powered by PLA technology, eliminates the need for traditional pre-coated capture antibodies and enables a fully homogeneous assay workflow. With no plate washing and no plate tapping required, the most labor-intensive steps of conventional immunoassays are completely removed.

By minimizing manual intervention throughout the assay, VeriQuant™ significantly reduces the risk of operator-induced errors and variability. There is no need to worry about residual wash buffer interference or inconsistencies caused by repeated handling. The result is greater assay robustness, superior reproducibility, and highly reliable data you can trust with confidence.
With VeriQuant™, every result is generated through a simpler, cleaner, and more consistent workflow—delivering dependable protein quantification without the burden of traditional washing steps.

H3:Ultra-Low Sample Input: Make Every Precious Sample Count

Have you ever had to postpone an experiment—or even repeat sample collection—simply because there wasn't enough material available?

Whether working with clinical specimens, limited-volume serum samples, rare biological fluids, or valuable cell culture supernatants, sample availability is often one of the biggest challenges in protein research. Unfortunately, traditional ELISA assays typically require 100 μL of sample per well, creating a significant barrier when sample volume is limited.

For many researchers, this means difficult compromises: fewer replicates, fewer targets tested, or in some cases, abandoning the experiment altogether.

The VeriQuant™ Immunoassay Kit changes that.

Leveraging the exceptional sensitivity of PLA technology, VeriQuant™ requires as little as 1 μL of sample to generate reliable quantitative results. This ultra-low sample requirement dramatically reduces the burden on researchers working with scarce or hard-to-obtain specimens.

Whether your samples come from small animal studies, limited clinical collections, primary cell cultures, or other precious biological sources, VeriQuant™ helps you maximize the value of every microliter.

Less sample consumption means more experiments, more data, and more opportunities for discovery.

When every sample matters, VeriQuant™ ensures that no precious specimen goes to waste.

H4:Ultra-Wide Dynamic Range: Measure More, Dilute Less

One of the most common frustrations with traditional ELISA is the need for extensive sample dilution optimization.
Because conventional ELISA assays typically offer a dynamic range of only two orders of magnitude, researchers often need to perform multiple pre-experiments to determine the appropriate dilution factor for each sample type. For biological samples with highly variable protein concentrations, this can mean repeated rounds of trial-and-error, additional reagent consumption, and significantly increased hands-on time.
Even worse, every dilution step introduces the possibility of pipetting errors and variability, potentially affecting the accuracy and reproducibility of the final results.

The VeriQuant™ Immunoassay Kit eliminates this challenge with an ultra-wide dynamic range exceeding five orders of magnitude.
From low-abundance biomarkers to highly expressed proteins, VeriQuant™ can accurately quantify a broad spectrum of concentrations within a single assay. Researchers no longer need to spend valuable time optimizing dilution factors or adjusting protocols for different sample types.
Wider range. Fewer dilutions. More reliable results.
By combining exceptional sensitivity with an industry-leading dynamic range, VeriQuant™ makes protein quantification easier, faster, and more accurate—even for the most challenging biological samples.

H5:Femtogram-Level Sensitivity: Capture Even the Lowest-Abundance Targets with Confidence

Detecting low-abundance proteins and trace biomarkers remains one of the greatest challenges in biological research. Conventional ELISA kits, with sensitivity typically limited to the picogram (pg) level, often struggle to accurately quantify weakly expressed targets, resulting in low signal intensity, undetectable analytes, and compromised data quality. These limitations can hinder the investigation of subtle biological changes and the discovery of novel biomarkers.

Powered by advanced signal amplification technology, the PLA Immunoassay Kit achieves femtogram (fg)-level sensitivity, representing a significant leap in detection performance. This ultra-high sensitivity enables the precise quantification of extremely low-abundance targets that would otherwise fall below the detection limits of traditional ELISA methods.

Whether you are studying rare proteins, exploring early-stage disease biomarkers, or investigating subtle molecular mechanisms, the PLA Immunoassay Kit provides the sensitivity required to reveal critical biological insights that might otherwise remain hidden.

Detect the undetectable. Reveal what conventional assays miss.

With femtogram-level sensitivity, the PLA Immunoassay Kit expands the boundaries of protein detection and empowers researchers to confidently tackle the most demanding applications in basic research, clinical biomarker discovery, and ultra-low-abundance target analysis.

From minimizing experimental variability and conserving precious samples to simplifying workflows, delivering femtogram-level sensitivity, and generating results faster than ever, the new VeriQuant™ Immunoassay Kit represents a comprehensive upgrade to traditional protein detection methods.

By eliminating cumbersome procedures and overcoming the inherent limitations of conventional ELISA, VeriQuant™ provides a more stable, efficient, and accurate approach to protein quantification. Designed to streamline laboratory workflows and accelerate scientific discovery, it offers researchers a powerful alternative to traditional ELISA without sacrificing data quality or reliability.

Empowering research through innovation. Enabling precise detection through simplicity.

With the new VeriQuant™ Immunoassay Kit, protein quantification becomes easier, faster, and more dependable—helping researchers spend less time troubleshooting assays and more time advancing breakthrough discoveries.