Catalog Number：VQ1064R
Rat REN VeriQuant Immunoassay Kit
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Size：
96 T
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MSDS
User Guide(a) Two monoclonal antibodies against distinct epitopes are conjugated with oligo probes, then form a sandwich complex with the antigen.
(b) Following antigen recognition by the paired antibodies, the conjugated oligonucleotides are spatially proximate, and generate an amplifiable dsDNA template under the enzymatic action.
(c) qPCR amplification is then performed. CT values correlate inversely with protein concentration, and sample target concentrations are calculated via standard curve.
Color-coded reagents components for easy identification
Kit outer packaging and components packaging
Product Operation Video
Product Details
| Product Name | Rat REN VeriQuant Immunoassay Kit | Species | Rat |
| Gene Alias | Ren; Ren1 | Gene ID | 24715 |
| Uniprot ID | P19677 | Protein Alias | Renin; Angiotensinogenase |
| Detection Method | Proximity-based Immunoassay | Sensitivity | 0.144pg/mL |
| Assay Instrument | qPCR Instruments | Detection Range | 0.256-4000pg/mL |
| Sample Type | Serum, Plasma, Tissue homogenates, Cell lysates, Cell culture supernates and Other biological fluids；Sample Volume=1~5μL | ||
| Total Assay Time | 2 H | Manual Handling Time | 20 min |
| Shipping Temperature | Ultra-low temperature phase-change materials | Storage Temperature | -20°C |
| No Washing Step | Yes | Tag or Dye | FAM™ |
| Kit Info | The Rat REN VeriQuant Immunoassay Kit is designed for the high-sensitivity quantitative measurement of Rat REN protein in minimal sample volumes. Utilizing our proprietary ReedLytix conjugation system coupled with Proximity Ligation Assay (PLA) technology, this kit integrates the specific antigen recognition of high-affinity antibodies with the exponential signal amplification power of qPCR. The result is a robust next-generation protein quantification platform. With a simple workflow, results can be obtained from sample to answer in just 2 hours. Data exported from your qPCR instrument can be directly analyzed via our online calculation tool at: https://www.reedbiotech.com/list/114.html ①Ultra-Sensitivity: All products achieve femtogram-level sensitivity ②Wide Range: Product detection range spans 6-log dynamic range ③Micro-Volume: Minimum sample size is only 1μL ④Fast: Results in 2 Hours(30 minutes of manual handling),no plate washing ⑤ALL IN ONE: Can replace traditional, high-sensitivity, micro-fast, fast-step, wide-range ELISA kits simultaneously |
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| Target Info | Renin is a member of the aspartyl proteinase family produced largely in part by the juxtaglomerular cells in the kidney. Renin is produced as prorenin with 43 pro residues at the N-terminal of mature Renin. The inactive prorenin becomes activated proteolytically by trypsin, cathepsin B, or other proteinases. Renin also has a very high selectivity for substrates due to a long peptide recognition on either side of the peptide bond undergoing cleavage. An octapeptide substrate was the minimum length to be cleaved by Renin. Renin plays a crucial role in the regulation of blood pressure and salt balance through the cleavage of angiotensinogen,which is the only known physiological substrate of Renin. Renin releases the decapeptide angiotensin I, which in turn is further converted to vasoactive hormone angiotensin II by angiotensin converting enzyme (ACE). | ||