Catalog Number：VQ1061H
Human VEGF VeriQuant Immunoassay Kit
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96 T
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MSDS
User Guide(a) Two monoclonal antibodies against distinct epitopes are conjugated with oligo probes, then form a sandwich complex with the antigen.
(b) Following antigen recognition by the paired antibodies, the conjugated oligonucleotides are spatially proximate, and generate an amplifiable dsDNA template under the enzymatic action.
(c) qPCR amplification is then performed. CT values correlate inversely with protein concentration, and sample target concentrations are calculated via standard curve.
Color-coded reagents components for easy identification
Kit outer packaging and components packaging
Product Operation Video
Product Details
| Product Name | Human VEGF VeriQuant Immunoassay Kit | Species | Human |
| Gene Alias | VEGFA, VEGF | Gene ID | 7422 |
| Uniprot ID | P15692 | Protein Alias | N-VEGF, VEGFA, Vascular permeability factor, VPF, L-VEGF, Vascular endothelial growth factor A, long form |
| Detection Method | Proximity-based Immunoassay | Sensitivity | 0.0704pg/mL |
| Assay Instrument | qPCR Instruments | Detection Range | 0.128-2000 pg/mL |
| Sample Type | Serum, Plasma, Tissue homogenates, Cell lysates, Cell culture supernates and Other biological fluids；Sample Volume=1~5μL | ||
| Total Assay Time | 2 H | Manual Handling Time | 20 min |
| Shipping Temperature | Ultra-low temperature phase-change materials | Storage Temperature | -20°C |
| No Washing Step | Yes | Tag or Dye | FAM™ |
| Kit Info | The Human VEGF VeriQuant Immunoassay Kit is designed for the high-sensitivity quantitative measurement of Human VEGF protein in minimal sample volumes. Utilizing our proprietary ReedLytix conjugation system coupled with Proximity Ligation Assay (PLA) technology, this kit integrates the specific antigen recognition of high-affinity antibodies with the exponential signal amplification power of qPCR. The result is a robust next-generation protein quantification platform. With a simple workflow, results can be obtained from sample to answer in just 2 hours. Data exported from your qPCR instrument can be directly analyzed via our online calculation tool at: https://www.reedbiotech.com/list/114.html ①Ultra-Sensitivity: All products achieve femtogram-level sensitivity ②Wide Range: Product detection range spans 6-log dynamic range ③Micro-Volume: Minimum sample size is only 1μL ④Fast: Results in 2 Hours(30 minutes of manual handling),no plate washing ⑤ALL IN ONE: Can replace traditional, high-sensitivity, micro-fast, fast-step, wide-range ELISA kits simultaneously |
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| Target Info | This protein is a member of the PDGF/VEGF growth factor family. It encodes a heparin-binding protein, which exists as a disulfide-linked homodimer. This growth factor induces proliferation and migration of vascular endothelial cells, and is essential for both physiological and pathological angiogenesis. Disruption of this gene in mice resulted in abnormal embryonic blood vessel formation. This protein is upregulated in many known tumors and its expression is correlated with tumor stage and progression. Elevated levels of this protein are found in patients with POEMS syndrome, also known as Crow-Fukase syndrome. | ||