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Catalog Number:RE2075H
Human Dyn(Big Dynorphin) ELISA Kit
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Size:

96 T

48 T

Price:

$399.00

$279.00

Delivery: Order now, ship in 3 days

Product Details
Product Name Human Dyn(Big Dynorphin) ELISA Kit Species Human
Uniprot ID P01213 Alternative Names Big dynorphin, Big Dyn
Detection method Competitive Sensitivity 4.69 pg/mL
Standard 500pg/mL Detection Range 7.82-500pg/mL
Sample type Serum, Plasma, Tissue homogenate and Other biological samples;Sample Volume=50μL
Reaction time 1.5H Research Area CD & Adhesion molecule;
Test principle This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with Human Dyn. During the reaction, Human Dyn in the sample or standard competes with a fixed amount of Human Dyn on the solid phase supporter for sites on the Biotinylated Detection Ab specific to Human Dyn. Excess conjugate and unbound sample or standard are washed away, and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns from blue to yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Human Dyn in tested samples can be calculated by comparing the OD of the samples to the standard curve.
Technical Data

As the OD values of the standard curve may vary according to the conditions of the actual assay performance (e.g. operator, pipetting technique, washing technique or temperature effects), the operator should establish a standard curve for each test. Typical standard curve and data is provided below for reference only.

(pg/mL) OD
500.00 0.167
250.00 0.305
125.00 0.524
62.50 0.855
31.25 1.276
15.63 1.733
7.82 2.042
0.00 2.522
Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level were tested 20 times on one plate, respectively.

Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level were tested on 3 different plates, 20 replicates in each plate, respectively.

Intra-assay Precision Inter-assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 20 20 20
Mean(pg/mL) 24.56 100.16 200.81 12.44 115.82 182.64
Standard deviation 0.51 5.52 7.21 0.16 4.94 8.51
C V (%) 5.43 4.48 3.59 3.29 4.72 5.67
Rate of recovery

The recovery of spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type Range(%) Average Recovery(%)
Serum (n=8) 90-105 97
EDTA plasma (n=8) 95-107 101
Cell culture media (n=8) 80-90 84
Linear

Samples were spiked with high concentrations of target proteins and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

Serum (n=5) EDTA plasma (n=5) Cell culture media (n=5)
1:2 Range (%) 98-107 81-94 96-105
Average (%) 106 86 99
1:4 Range (%) 90-99 81-94 82-94
Average (%) 94 92 93
1:8 Range (%) 88-97 87-105 95-102
Average (%) 92 96 98
1:16 Range (%) 85-97 80-95 87-103
Average (%) 96 87 87
Assay Procedures