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Catalog Number:RE2014H
Human MPA(Mycophenolic Acid) ELISA Kit
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Size:

96 T

48 T

Price:

$399.00

$279.00

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Product Details
Product Name Human MPA(Mycophenolic Acid) ELISA Kit Species Human
Uniprot ID N/A Alternative Names mycophenolate
Detection method Competitive Sensitivity 18.75 pg/mL
Standard 2000pg/mL Detection Range 31.25-2000pg/mL
Sample type Serum, Plasma, Tissue homogenate and Other biological samples;Sample Volume=50μL
Reaction time 1.5H Research Area Enzyme & Kinase;
Test principle This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with Human MPA. During the reaction, Human MPA in the sample or standard competes with a fixed amount of Human MPA on the solid phase supporter for sites on the Biotinylated Detection Ab specific to Human MPA. Excess conjugate and unbound sample or standard are washed away, and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns from blue to yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Human MPA in tested samples can be calculated by comparing the OD of the samples to the standard curve.
Technical Data

As the OD values of the standard curve may vary according to the conditions of the actual assay performance (e.g. operator, pipetting technique, washing technique or temperature effects), the operator should establish a standard curve for each test. Typical standard curve and data is provided below for reference only.

(pg/mL) OD
2000.00 0.182
1000.00 0.318
500.00 0.533
250.00 0.867
125.00 1.295
62.50 1.687
31.25 2.063
0.00 2.503
Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level were tested 20 times on one plate, respectively.

Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level were tested on 3 different plates, 20 replicates in each plate, respectively.

Intra-assay Precision Inter-assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 20 20 20
Mean(pg/mL) 20.88 301.02 967.42 46.46 321.03 735.72
Standard deviation 4.63 23.98 45.85 5.22 13.10 44.41
C V (%) 5.03 5.56 6.11 4.19 4.21 7.03
Rate of recovery

The recovery of spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type Range(%) Average Recovery(%)
Serum (n=8) 80-92 85
EDTA plasma (n=8) 92-106 99
Cell culture media (n=8) 87-95 91
Linear

Samples were spiked with high concentrations of target proteins and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

Serum (n=5) EDTA plasma (n=5) Cell culture media (n=5)
1:2 Range (%) 83-97 87-96 81-90
Average (%) 89 88 90
1:4 Range (%) 82-96 87-96 82-96
Average (%) 92 93 95
1:8 Range (%) 86-98 86-95 85-98
Average (%) 97 91 96
1:16 Range (%) 93-101 92-103 92-103
Average (%) 100 102 93
Assay Procedures