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Catalog Number:RE2458MF
MF-Mouse TNFRSF1B(Tumor Necrosis Factor Receptor Superfamily, Member 1B) ELISA Kit
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Size:

48 T

96 T

Price:

$329.00

$459.00

Delivery: Order now, ship in 3 days

Product Details
Product Name MF-Mouse TNFRSF1B(Tumor Necrosis Factor Receptor Superfamily, Member 1B) ELISA Kit Species Mouse
Uniprot ID P25119 Alternative Names TNFR2, CD12b, TBPII, TNF-R-II, TNF-R75, TNFBR, TNFR1B, TNFR8, p75, p75TNFR
Detection method Sandwich Sensitivity 4.69 pg/mL
Standard 500pg/mL Detection Range 7.82-500pg/mL
Sample type Serum, Plasma and Tissue homogenate and Other biological samples;Sample volume:25μL
Reaction time 3H Research Area Signaling transduction, Cell biology, Neuroscience, Cancer, Immunology;
Test principle This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse TNFRSF1B. Samples (or Standards) are added to the micro ELISA plate wells and combined with the specific antibody and biotinylated detection antibody specific for Mouse TNFRSF1B. Then Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse TNFRSF1B, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm. The OD value is proportional to the concentration of Mouse TNFRSF1B. You can calculate the concentration of Mouse TNFRSF1B in the samples by comparing the OD of the samples to the standard curve.
Technical Data

As the OD values of the standard curve may vary according to the conditions of the actual assay performance (e.g. operator, pipetting technique, washing technique or temperature effects), the operator should establish a standard curve for each test. Typical standard curve and data is provided below for reference only.

(pg/mL) OD Corrected
500.00 2.458 2.435
250.00 1.602 1.579
125.00 0.936 0.913
62.50 0.417 0.394
31.25 0.208 0.185
15.63 0.110 0.087
7.82 0.058 0.035
0.00 0.023 0.000
Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level were tested 20 times on one plate, respectively.

Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level were tested on 3 different plates, 20 replicates in each plate, respectively.

Intra-assay Precision Inter-assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 20 20 20
Mean(pg/mL) 10.98 77.16 195.370 12.61 95.560 184.750
Standard deviation 0.56 3.36 6.57 0.58 5.40 11.09
C V (%) 4.66 3.60 5.73 4.47 3.89 3.40
Rate of recovery

The recovery of spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type Range(%) Average Recovery(%)
Serum (n=8) 80-95 87
EDTA plasma (n=8) 88-102 95
Cell culture media (n=8) 90-105 97
Linear

Samples were spiked with high concentrations of target proteins and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

Serum (n=5) EDTA plasma (n=5) Cell culture media (n=5)
1:2 Range (%) 87-101 95-102 85-92
Average (%) 98 96 86
1:4 Range (%) 87-96 95-102 88-95
Average (%) 90 99 92
1:8 Range (%) 82-93 83-96 96-102
Average (%) 83 87 102
1:16 Range (%) 89-97 97-103 89-103
Average (%) 90 98 90
Assay Procedures