Catalog Number：RA10386
NFκB p65 Mouse Monoclonal Antibody(5G6)
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Size：
50 μL
100 μL
Price：
$198.00
$298.00
Delivery： Order now, ship in 3 days
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| Product Name | NFκB p65 Mouse Monoclonal Antibody(5G6) | Species | H, R, M |
| Applications | WB, IP, IF, IHC | ||
| Condition | PBS, pH 7.4, containing 0.02% sodium azide as Preservative and 50% Glycerol. | ||
| Alternative Names | NF kappa B p65, NFKB3, p65, p65 NF kB, RELA, TF65, Transcription Factor p65 | ||
| Store | -20°C. Do not aliquot the antibody. | ||
| Recommended dilutions | WB: 1:500-2,000 IP:1:200 IF: 1:100-200 IHC:1:200-500 Optimal dilutions should be determined by the end user. | ||
| Specificity | NFκB p65 Mouse monoclonal antibody detects endogenous p65 proteins. | ||
| Background | NFkB p65 is ubiquitinated leading to its proteosomal degradation, which is required for termination of the NFkB response. Phosphorylation of NFkB p65 on S536 stimulates acetylation of K310 by CBP, enhancing transcriptional activity. NFkB p65 is also acetylated at K122, enhancing DNA binding and impairing the interaction with NFKBIA. The protein is deacetylated by HDAC3. Invasion of a host by a pathogen is frequently associated with the activation of NF-kB, which coordinates various aspects of immune function required for resistance to infection. | ||
| Molecular Weight | 65KD | Isotype | IgG1 |
| GeneID | 5970 | Protein MW | 65 |
| SWISS | Q04206 | Source | Mouse |
| Antigen | Recombinant Protein | Category | Monoclonal |
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Western blot analysis of 1) Hela, 2) Rat Heart Tissue, 3) Mouse Spleen Tissue with NFkB p65 Mouse mAb diluted at 1:2000.
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Immunohistochemical analysis of paraffin-embedded Rat Brain Tissue using NFkB p65 Mouse mAb diluted at 1:500.