Catalog Number：RA10246
NFkB p65 Mouse Monoclonal Antibody (14H2)
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Size：
50 μL
100 μL
Price：
$198.00
$298.00
Delivery： Order now, ship in 3 days
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| Product Name | NFkB p65 Mouse Monoclonal Antibody (14H2) | Species | H,M,R |
| Applications | WB, IHC, IP | ||
| Condition | PBS, pH 7.4, containing 0.02% sodium azide as Preservative and 50% Glycerol. | ||
| Alternative Names | |||
| Store | -20°C. Do not aliquot the antibody. | ||
| Recommended dilutions | WB: 1:1,000-3,000 IHC: 1:200 IP:1:200 Optimal dilutions should be determined by the end user. | ||
| Specificity | NFκB p65 Mouse monoclonal antibody detects endogenous p65 proteins. | ||
| Background | NFkB p65 is ubiquitinated leading to its proteosomal degradation, which is required for termination of the NFkB response. Phosphorylation of NFkB p65 on S536 stimulates acetylation of K310 by CBP, enhancing transcriptional activity. NFkB p65 is also acetylated at K122, enhancing DNA binding and impairing the interaction with NFKBIA. The protein is deacetylated by HDAC3. Invasion of a host by a pathogen is frequently associated with the activation of NF-kB, which coordinates various aspects of immune function required for resistance to infection. | ||
| Molecular Weight | ~65KD | Isotype | IgG1 |
| GeneID | 5970 | Protein MW | 65 |
| SWISS | Q04206 | Source | Mouse |
| Antigen | Synthetic Peptide | Category | Monoclonal |
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Western blot analysis of 1) Hela, 2)293T,3)HepG with NFκB p65 mAb diluted at 1:2,000.
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IHC staining of mouse hippocampus tissue with NFκB p65 mouse mAb(14H2) diluted at 1:200.