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Catalog Number:RE2557R
Rat GPX1(Glutathione Peroxidase 1) ELISA Kit
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Size:

96 T

48 T

Price:

$399.00

$279.00

Delivery: Order now, ship in 3 days

Product Details
Product Name Rat GPX1(Glutathione Peroxidase 1) ELISA Kit Species Rat
Uniprot ID P04041 Alternative Names GPX1, GPXD, GSHPX1, glutathione peroxidase 1
Detection method Sandwich Sensitivity 18.75 pg/mL
Standard 2000pg/mL Detection Range 31.25-2000pg/mL
Sample type Serum, Plasma, Tissue homogenate and Other biological samples;Sample Volume=100μL
Reaction time 3.5H Research Area Cytokine;Infection immunity;
Test principle This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat GPX1. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat GPX1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat GPX1, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat GPX1. You can calculate the concentration of Rat GPX1 in the samples by comparing the OD of the samples to the standard curve.
Technical Data

As the OD values of the standard curve may vary according to the conditions of the actual assay performance (e.g. operator, pipetting technique, washing technique or temperature effects), the operator should establish a standard curve for each test. Typical standard curve and data is provided below for reference only.

(pg/mL) OD Corrected
2000.00 2.345 2.284
1000.00 1.551 1.490
500.00 0.920 0.859
250.00 0.437 0.376
125.00 0.258 0.197
62.50 0.158 0.097
31.25 0.111 0.050
0.00 0.061 0.000
Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level were tested 20 times on one plate, respectively.

Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level were tested on 3 different plates, 20 replicates in each plate, respectively.

Intra-assay Precision Inter-assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 20 20 20
Mean(pg/mL) 54.80 367.91 663.40 50.57 458.01 767.34
Standard deviation 2.81 14.83 34.61 2.83 24.56 37.53
C V (%) 3.40 4.33 6.25 6.53 7.79 4.06
Rate of recovery

The recovery of spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type Range(%) Average Recovery(%)
Serum (n=8) 83-96 89
EDTA plasma (n=8) 92-107 99
Cell culture media (n=8) 88-102 95
Linear

Samples were spiked with high concentrations of target proteins and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

Serum (n=5) EDTA plasma (n=5) Cell culture media (n=5)
1:2 Range (%) 95-102 87-97 89-98
Average (%) 96 88 93
1:4 Range (%) 83-96 87-97 90-99
Average (%) 91 93 91
1:8 Range (%) 97-103 86-92 95-106
Average (%) 101 92 101
1:16 Range (%) 85-92 89-103 87-101
Average (%) 86 101 87
Assay Procedures