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Catalog Number:RE1241H
Human IDE(Insulin Degrading Enzyme) ELISA Kit
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Size:

96 T

48 T

Price:

$399.00

$279.00

Delivery: Order now, ship in 3 days

Product Details
Product Name Human IDE(Insulin Degrading Enzyme) ELISA Kit Species Human
Uniprot ID P14735 Alternative Names Insulysin, Insulin protease
Detection method Sandwich Sensitivity 37.5 pg/mL
Standard 4000pg/mL Detection Range 62.5-4000pg/mL
Sample type Plasma;Sample Volume=100μL
Reaction time 3.5H Research Area Immune molecule;
Test principle This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human IDE. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human IDE and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human IDE, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human IDE. You can calculate the concentration of Human IDE in the samples by comparing the OD of the samples to the standard curve.
Technical Data

As the OD values of the standard curve may vary according to the conditions of the actual assay performance (e.g. operator, pipetting technique, washing technique or temperature effects), the operator should establish a standard curve for each test. Typical standard curve and data is provided below for reference only.

(pg/mL) OD Corrected
4000.00 2.391 2.334
2000.00 1.551 1.494
1000.00 0.877 0.820
500.00 0.477 0.420
250.00 0.257 0.200
125.00 0.154 0.097
62.50 0.107 0.050
0.00 0.057 0.000
Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level were tested 20 times on one plate, respectively.

Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level were tested on 3 different plates, 20 replicates in each plate, respectively.

Intra-assay Precision Inter-assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 20 20 20
Mean(pg/mL) 119.61 946.79 1818.88 108.51 610.82 1495.55
Standard deviation 5.78 25.31 54.17 5.42 47.54 66.88
C V (%) 3.94 3.45 4.30 5.91 6.84 3.53
Rate of recovery

The recovery of spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type Range(%) Average Recovery(%)
Serum (n=8) 95-107 101
EDTA plasma (n=8) 96-104 100
Cell culture media (n=8) 98-104 102
Linear

Samples were spiked with high concentrations of target proteins and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

Serum (n=5) EDTA plasma (n=5) Cell culture media (n=5)
1:2 Range (%) 89-99 86-97 96-105
Average (%) 91 88 96
1:4 Range (%) 88-104 86-97 95-102
Average (%) 98 90 102
1:8 Range (%) 84-98 83-96 98-106
Average (%) 96 94 104
1:16 Range (%) 95-105 91-103 87-98
Average (%) 95 102 90
Assay Procedures