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Catalog Number:RE2184R
Rat MT(Melatonin) ELISA Kit
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Size:

96 T

48 T

Price:

$359.00

$259.00

Delivery: Order now, ship in 3 days

Product Details
Product Name Rat MT(Melatonin) ELISA Kit Species Rat
Uniprot ID N/A Alternative Names N-Acetyl-5-Methoxytryptamine
Detection method Competitive Sensitivity 9.38 pg/mL
Standard 1000pg/mL Detection Range 15.63-1000pg/mL
Sample type Serum, Brain dialysate;Sample Volume=50μL
Reaction time 1.5H Research Area Kidney diseases
Test principle This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with Rat MT. During the reaction, Rat MT in the sample or standard competes with a fixed amount of Rat MT on the solid phase supporter for sites on the Biotinylated Detection Ab specific to Rat MT. Excess conjugate and unbound sample or standard are washed away, and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns from blue to yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Rat MT in tested samples can be calculated by comparing the OD of the samples to the standard curve.
Technical Data

As the OD values of the standard curve may vary according to the conditions of the actual assay performance (e.g. operator, pipetting technique, washing technique or temperature effects), the operator should establish a standard curve for each test. Typical standard curve and data is provided below for reference only.

(pg/mL) OD
1000.00 0.264
500.00 0.364
250.00 0.558
125.00 0.819
62.50 1.114
31.25 1.509
15.63 1.823
0.00 2.487
Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level were tested 20 times on one plate, respectively.

Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level were tested on 3 different plates, 20 replicates in each plate, respectively.

Intra-assay Precision Inter-assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 20 20 20
Mean(pg/mL) 48.87 130.91 386.32 51.46 151.47 327.28
Standard deviation 0.59 10.09 15.29 2.68 11.74 23.48
C V (%) 3.18 6.74 2.87 8.01 3.11 4.95
Rate of recovery

The recovery of spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type Range(%) Average Recovery(%)
Serum (n=8) 88-103 95
EDTA plasma (n=8) 92-106 99
Cell culture media (n=8) 90-105 97
Linear

Samples were spiked with high concentrations of target proteins and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

Serum (n=5) EDTA plasma (n=5) Cell culture media (n=5)
1:2 Range (%) 88-103 95-106 93-102
Average (%) 90 106 102
1:4 Range (%) 83-94 95-106 90-99
Average (%) 89 96 96
1:8 Range (%) 92-105 88-103 95-106
Average (%) 101 90 100
1:16 Range (%) 89-97 81-98 87-101
Average (%) 97 89 100
Assay Procedures