PRODUCTS

Catalog Number:RE2388M
Mouse TFR2(Transferrin Receptor 2) ELISA Kit
Copy Product Information

Size:

96 T

48 T

Price:

$359.00

$259.00

Delivery: Order now, ship in 3 days

Product Details
Product Name Mouse TFR2(Transferrin Receptor 2) ELISA Kit Species Mouse
Uniprot ID Q9JKX3 Alternative Names HFE3, TFRC2
Detection method Sandwich Sensitivity 0.94 ng/mL
Standard 100ng/mL Detection Range 1.57-100ng/mL
Sample type Serum, Plasma, Tissue homogenate and Other biological samples;Sample Volume=100μL
Reaction time 3.5H Research Area Signal transduction;Tumor immunity;
Test principle This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse TFR2. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse TFR2 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse TFR2, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse TFR2. You can calculate the concentration of Mouse TFR2 in the samples by comparing the OD of the samples to the standard curve.
Technical Data

As the OD values of the standard curve may vary according to the conditions of the actual assay performance (e.g. operator, pipetting technique, washing technique or temperature effects), the operator should establish a standard curve for each test. Typical standard curve and data is provided below for reference only.

(ng/mL) OD Corrected
100.00 2.348 2.311
50.00 1.546 1.509
25.00 0.943 0.906
12.50 0.511 0.474
6.25 0.256 0.219
3.13 0.141 0.104
1.57 0.080 0.043
0.00 0.037 0.000
Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level were tested 20 times on one plate, respectively.

Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level were tested on 3 different plates, 20 replicates in each plate, respectively.

Intra-assay Precision Inter-assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 20 20 20
Mean(ng/mL) 1.77 12.52 39.11 2.97 15.52 27.85
Standard deviation 0.14 1.24 2.33 0.09 1.06 2.53
C V (%) 2.87 3.00 4.90 2.62 6.16 6.61
Rate of recovery

The recovery of spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type Range(%) Average Recovery(%)
Serum (n=8) 80-94 86
EDTA plasma (n=8) 90-103 96
Cell culture media (n=8) 81-96 88
Linear

Samples were spiked with high concentrations of target proteins and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

Serum (n=5) EDTA plasma (n=5) Cell culture media (n=5)
1:2 Range (%) 81-94 80-101 95-103
Average (%) 94 88 101
1:4 Range (%) 96-105 80-101 85-92
Average (%) 98 100 86
1:8 Range (%) 92-103 87-96 80-96
Average (%) 99 95 87
1:16 Range (%) 82-96 96-105 95-103
Average (%) 86 97 101
Assay Procedures