PRODUCTS

Catalog Number:RE2069M
Mouse PGF2α(Prostaglandin F2 Alpha) ELISA Kit
Copy Product Information

Size:

96 T

48 T

Price:

$399.00

$279.00

Delivery: Order now, ship in 3 days

Product Details
Product Name Mouse PGF2α(Prostaglandin F2 Alpha) ELISA Kit Species Mouse
Uniprot ID N/A Alternative Names Amoglandin, Croniben, Cyclosin, Dinifertin, Enzaprost, Glandin, PGF2α, Panacelan, Prostamodin
Detection method Competitive Sensitivity 9.38 pg/mL
Standard 1000pg/mL Detection Range 15.63-1000pg/mL
Sample type Serum, Plasma, Tissue homogenate and Other biological samples;Sample Volume=50μL
Reaction time 1.5H Research Area Endocrinology;Reproductive science;Hormone metabolism;
Test principle This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with Mouse PGF2α. During the reaction, Mouse PGF2α in the sample or standard competes with a fixed amount of Mouse PGF2α on the solid phase supporter for sites on the Biotinylated Detection Ab specific to Mouse PGF2α. Excess conjugate and unbound sample or standard are washed away, and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns from blue to yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Mouse PGF2α in tested samples can be calculated by comparing the OD of the samples to the standard curve.
Technical Data

As the OD values of the standard curve may vary according to the conditions of the actual assay performance (e.g. operator, pipetting technique, washing technique or temperature effects), the operator should establish a standard curve for each test. Typical standard curve and data is provided below for reference only.

(pg/mL) OD
1000.00 0.275
500.00 0.375
250.00 0.569
125.00 0.830
62.50 1.125
31.25 1.520
15.63 1.834
0.00 2.498
Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level were tested 20 times on one plate, respectively.

Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level were tested on 3 different plates, 20 replicates in each plate, respectively.

Intra-assay Precision Inter-assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 20 20 20
Mean(pg/mL) 4.72 234.92 308.93 34.98 213.43 472.17
Standard deviation 0.66 11.08 23.39 1.50 9.92 21.01
C V (%) 2.98 6.02 6.53 5.77 5.79 3.35
Rate of recovery

The recovery of spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type Range(%) Average Recovery(%)
Serum (n=8) 85-97 91
EDTA plasma (n=8) 81-95 88
Cell culture media (n=8) 90-105 97
Linear

Samples were spiked with high concentrations of target proteins and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

Serum (n=5) EDTA plasma (n=5) Cell culture media (n=5)
1:2 Range (%) 85-95 83-103 81-96
Average (%) 87 99 84
1:4 Range (%) 80-96 83-103 92-106
Average (%) 84 102 106
1:8 Range (%) 89-101 82-93 92-101
Average (%) 101 90 96
1:16 Range (%) 85-97 86-95 80-95
Average (%) 96 87 83
Assay Procedures