Catalog Number：REWB101
Hypersensitivity ECL Chemiluminescence Detection Kit
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Size：
100mL
Price：
$588.00
Delivery： spots
Product Details
| Product Name | Hypersensitivity ECL Chemiluminescence Detection Kit | component1 | Solution A 50ML |
| component2 | 溶液B 50ML | component3 | / |
| component4 | / | Storage | Sealed and stored in a dark place at 4 ℃, valid for one year |
| Description | The hypersensitive ECL chemiluminescence detection kit is a substrate detection kit used together with Horseradish peroxidase (HRP) in Western blot experiments. It can be used to detect Horseradish peroxidase (HRP) labeled proteins or nucleic acids, and to analyze protein content by protein imprinting or ELISA. It is compatible with film and digital development systems. | ||
| Instructions | 1. According to the requirements of immunoblotting experiments, seal the imprinting membrane and incubate the primary antibody and corresponding secondary antibody. 2. Wash the film with TBST three times, each time for 5 minutes. 3. Take an appropriate amount of substrate A and substrate B and mix them in a 1:1 ratio as needed. Note: The reagent is currently prepared and used, and the suction head of solution A and solution B must be replaced during the suction process. 4. Take an appropriate amount of mixed substrate solution and add it to the membrane. 5. Adjust the exposure time based on the intensity of the luminescence. | ||
| Cautions | "1. During the absorption of liquid A and liquid B, the gun head of the Pipette must be replaced to avoid mutual pollution and failure. 2. After liquid A and liquid B are mixed into the working liquid, the influence of strong oxidants such as Sodium hypochlorite on the working liquid should be avoided. 3. It is necessary to ensure that the reagent bottle is clean and free from microbial or other reagent contamination. 4. For your safety, please wear White coat and disposable gloves. “ | ||