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Catalog Number：RCK001
Ultra Cell Counting Kit-8(CCK-8)Assay Kit
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500 T

10000 T

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Product Details
Product Name Ultra Cell Counting Kit-8(CCK-8)Assay Kit Applications Cell Multiplication
Detection principle In the presence of the electron mediator 1-Methoxy PMS, WST-8 can be reduced by certain mitochondrial dehydrogenases to produce a water-soluble orange-yellow formazan (see Figure 1). The intensity of the formazan color is directly proportional to cell proliferation and inversely proportional to cytotoxicity. The optical density (OD) is measured at 450 nm using a microplate reader, which indirectly reflects the number of living cells. Compared to the basic CCK-8 kit, this ultrasensitive version offers higher sensitivity, significantly stronger signal, and rapid reaction (most cells show noticeable color change after approximately 30 minutes of incubation). It also has a broader linear range, effectively improving experimental efficiency. It is suitable for assays of cell proliferation, cytotoxicity, drug screening, and inhibition of cell growth.
Detection method Colorimetric method Sample type Cell
Detection time 0.5-2 Hours Testing instrument Microplate Reader
fluorescence N/A Ex/Em (nm) 450nm
Channel set N/A Self-supplied reagent N/A
Storage temperature 2~8°C, shading light Transport condition Blue ice
Shelf life 12 months