Please predict the concentration range of the sample in advance. If your samples need to be diluted, please refer to the following dilution instructions:
For 100 folds dilution: One step dilution. Add 5μL sample to 495μL sample diluent to yield 100 folds dilution.
For 1000 folds dilution: Two step dilution. Add 5μL sample to 95μL sample diluent to yield 20 folds dilution, then add 5μL 20 folds diluted sample to 245μL sample diluent, after this, the neat sample has been diluted at 1000 folds successfully.
For 100,000 folds dilution: Three step dilution. Add 5μL sample to 195μL sample diluent to yield 40 folds dilution, then add 5μL 40 folds diluted sample to 245μL sample diluent to yield 50 folds dilution, and finally add 5μL 2000 folds diluted sample to 245μL sample diluent, after this, the neat sample has been diluted at 100,000 folds successfully.